Lipase-catalyzed transesterification of medium-long-medium structured lipid (MLM-SL) using palm olein and tricaprylin in packed-bed reactor (PBR)

Lipase-catalyzed transesterification between refined bleached deodorized palm olein (RBDO) and tricaprylin to produce medium-long-medium structured lipid (MLM-SL) in a packed bed reactor (PBR) has been investigated. A specific sn-1,3 commercial Lipozyme TL IM was used as biocatalyst. Within this study, the progress of monitored especially for triacylglycerol (TAG) formation with equivalent carbon number (ECN) 32, presumably 1,3-dicapryoyl-2-oleoyl-sn-glycerol (COC). Transesterification conditions investigated were residence times (i.e., 15, 30, 60 min) enzyme loadings (2.0 4.5 g). The highest yield ECN 32 (13%) degree (71%) obtained at time 15 mins both loadings. Longer seemed facilitate hydrolysis over transesterification. This indicated by peaks appearing high-performance liquid chromatography (HPLC) chromatograms reduction fat slip melting point (SMP). Additionally, productivity 2.0 g loading. Conclusively, study demonstrated potential use packed-bed immobilized continuous synthesis MLM-SLs TAG ECN32.